Postnatal gestational age dating methods have been needed in low and middle income settings because ultrasound is often unavailable, last menstrual dates are uncertain, and physical and neurological scoring is complex. The disappearance of anterior lens capsule vascularity (ALCV), a normal embryological process, has a high correlation with gestational age at delivery among preterm neonates. So, the primary aim of this project was to establish the validity of smartphone ophthalmoscope ALCV gestational age estimates among preterm neonates at delivery compared with the referent standard of ultrasound gestational age. To carry out this aim RTI used a smartphone ophthalmoscope to capture images of ALCV in preterm neonates, developed an ALCV biomarker dataset using image analysis software, and developed and validated a predictive algorithm for gestational age using ALCV image biomarkers.