RTI International developed a novel, highly automated method of gestational age estimation at delivery combining anterior lens capsule vascularity (ALCV) and biophysical parameters appropriate for use in low-income countries. The specific aims of the study are: (1) to develop an algorithm to predict gestational age at delivery from 26 to 42 weeks’ gestation with ALCV and key biophysical parameters and (2) to evaluate the performance of gestational age estimates based on ALCV and biophysical parameters. RTI accomplished this through a multi-site, cross-sectional study among newborn children in the United States and internationally to measure ALCV and key biophysical measures of gestational age, and, via modern statistical approaches, to develop a robust predictive algorithm for gestational age. The combination of domestic sites allowed the project team to test and refine the algorithm in U.S. research universities, while the international sites allowed the team to explore feasibility and performance in low-resource settings.